Features of Histomorphology of the Pituitary Gland, Spinal Cord and Cerebral in Cattle

The anatomical, morphological, neuro-histological and morphometric research methods were used for outlining the features of the histological structure of the pituitary gland, spinal cord and cerebellum of cattle in the article. For histological and neurohistological examinations, pieces of material were fixed in a 12 % aqueous solution of neutral formalin, followed by paraffin filling, after which serial sections were made, which were stained with hematoxylin and eosin. Impregnation with silver nitrate was also performed according to the Bilshovskym-Gross method. The general histological structure (histo- and cytostructure) of organs in histological specimens was studied under a light microscope. This investigation with domestic animals was guided by the "European Convention for the Protection of Vertebrate Animals Used for Experimental and Other Scientific Purposes" (Strasbourg, 1986).

Based on morphometric studies, different thicknesses of the pituitary and cerebellar cortex of the cattle were established. The quantitative characteristics of the neural composition and the ratio of nerve cell populations in the structure of the gray matter of the spinal cord of cattle are given, which indicating a pronounced differentiation of nerve cells that have different shapes and sizes and, accordingly, different nuclear-cytoplasmic ratios.